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KMID : 0545120060160101591
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Journal of Microbiology and Biotechnology
2006 Volume.16 No. 10 p.1591 ~ p.1596
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Color Alteration and Acaricidal Activity of Juglone Isolated from Caesalpinia sappan Heartwoods Against Dermatophagoides spp.
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Lee Chi-Hoon
Lee Hoi-Seon
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Abstract
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Acaricidal effects of materials derived from Caesalpinia sappan heartwoods against Dermatophagoides farinae and D. pteronyssinus were assessed and compared with those evidenced by commercial benzyl benzoate and DEET. The observed responses varied according to dosage and mite species. The values of the methanol extracts derived from C. sappan heartwoods were 6.13 and against D. farinae and D. pteronyssinus, respectively. Furthermore, the ethyl acetate fraction derived from the methanol extract was approximately 8.71 more toxic than DEET against D. farinae, and 4.73 times more toxic against D. pteronyssinus. The biologically active constituent from the ethyl acetate fraction of C. sappan heartwood extract was purified via silica gel chromatography and high-performance liquid chromatography. The structure of the acaricidal component was analyzed by , and DEPT-NMR spectroscopy, and identified as juglone (5-hydroxy-l,4-naphthoquinone). Based on the values of juglone and its derivatives, the most toxic compound against D. farinae was juglone (), followed by benzyl benzoate () and 2methyl-l,4-naphthoquinone (). These results indicate that the acaricidal activity of C. sappan heartwoods is likely to be the result of the effects of juglone. Additionally, juglone treatment was shown to effect a change in the color of the cuticles of house dust mites, from colorless-transparent to dark brownish-black. Accordingly, as a naturally occurring acaricidal agent, C. sappan heartwood-derived juglone should prove to be quite 'useful as a potential control agent, lead compound, and house dust mite indicator.
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KEYWORD
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Caesalpinia sappan, Dermatophagoides farinae, Dermatophagoides pteronyssinus, indicator, juglone
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